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Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
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Biologia
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JoVE Journal Biologia
Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

DOI:
10.3791/64922-v

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07:46 min

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July 28, 2023

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, , , ,
1Faculty of Biology,Ludwig Maximilian University of Munich, 2Institute for the Advanced Study of Human Biology,Kyoto University, 3Hakubi Center,Kyoto University

Capítulos

  • 00:04Introduction
  • 00:56Isolation of Cells from Urine Samples
  • 01:58Generation of Induced‐PSCs by Sendai Virus Vector Infection
  • 03:50Clump‐Passaging
  • 05:15Results: Generation and Characterization of Induced‐PSCs
  • 06:59Conclusion

Summary

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Tadução automática

The present protocol describes a method to isolate, expand, and reprogram human and non-human primate urine-derived cells to induced pluripotent stem cells (iPSCs), as well as instructions for feeder-free maintenance of the newly generated iPSCs.

Tags

PrimateInduced Pluripotent Stem CellsUrinary CellsNon-invasiveSendai VirusReprogrammingBasement Membrane MatrixREMC MediumCentrifugationCell Culture

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