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TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis
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JoVE Journal Neurociência
TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

DOI:
10.3791/64953-v

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09:39 min

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December 16, 2022

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, , , , ,
1School of Neuroscience,Virginia Polytechnic Institute and State University, 2CMU-Pitt Joint Computational Biology, School of Medicine,University of Pittsburgh, 3Eastern Virginia Medical School, 4Department of Physics,University of Miami

Capítulos

  • 00:05Introduction
  • 00:33Calcium Imaging
  • 01:38Analysis of 3D Calcium Imaging Data by TACI
  • 07:29Results: Using TACI to Analyze 3D Calcium Imaging
  • 08:48Conclusion

Summary

Tadução automática

Tadução automática

TrackMate Analysis of Calcium Imaging (TACI) is an open-source ImageJ plugin for 3D calcium imaging analysis that examines motion on the z-axis and identifies the maximum value of each z-stack to represent a cell's intensity at the corresponding time point. It can separate neurons overlapping in the lateral (x/y) direction but on different z-planes.

Tags

Keywords: 3D Calcium ImagingImageJ PluginTACIMotion CorrectionZ-driftTemperature RecordingPeltierTIFF File RenamingTIFF File Organizing

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