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Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

DOI:
10.3791/64971-v

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09:39 min

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June 02, 2023

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, ,
1Department of Immunology, School of Medicine,UConn Health, 2Arthritis and Clinical Immunology Program,Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology,University of Oklahoma Health Science Center

Capítulos

  • 00:05Introduction
  • 01:05Preparation of the 96-Well Plate for the Metabolic Extracellular Flux Assay
  • 01:52Preparation and Seeding of Cells
  • 03:52Preparing Compounds in the Mitochondrial Stress Test Kit
  • 04:25Running the Mitochondrial Stress Assay
  • 07:29Results: Metabolic Flux Assay to Analyze Oxygen Consumption Rates (OCR) in Neutrophils
  • 09:02Conclusion

Summary

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We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer.

Tags

Mitochondrial RespirationNeutrophilsHL60 CellsMetabolic Extracellular Flux AnalyzerMitochondrial FunctionCell Seeding DensityAssay MediumMouse NeutrophilsHuman NeutrophilsDifferentiated HL60 CellsUndifferentiated HL60 CellsBackground Correction

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