/

/

氯化铁诱导的动脉血栓形成和用于3D电子显微镜分析的样本采集

JoVE Journal
Medicina

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Medicina

Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis

Please note that all translations are automatically generated. Click here for the English version.

氯化铁诱导的动脉血栓形成和用于3D电子显微镜分析的样本采集

DOI:

10.3791/64985-v

•

03:57 min

•

March 17, 2023

•

Smita Joshi*¹, Alexis N. Smith, Kanakanagavalli Shravani Prakhya, Hammodah R. Alfar, Joshua Lykins, Ming Zhang, Irina Pokrovskaya, Maria Aronova, Richard D. Leapman, Brian Storrie, Sidney W. Whiteheart*¹

¹Department of Molecular and Cellular Biochemistry,University of Kentucky, ²Department of Physiology and Cell Biology,University of Arkansas for Medical Sciences, ³Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health

Capítulos

00:05Introduction
00:48Sample Preparation for Serial Block Face Scanning Electron Microscopy Studies Post Ferric Chloride‐Induced Injury
02:47Results: Ferric Chloride‐Mediated Injury to Induce Arterial Thrombosis
03:29Conclusion

Summary

Tadução automática

本协议描述了如何使用FeCl₃介导的损伤来诱导动脉血栓形成，以及如何在血栓形成的各个阶段收集和制备动脉损伤样品以进行电子显微镜分析。

Tags

Keywords: Ferric Chloride-Induced Arterial Thrombosis 3D Electron Microscopy Analysis Platelets Hemostatic System Thrombosis Carotid Artery Iron Chloride Injury Flow Measurements Fixative Electron Microscopy Blood Flow

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

注射原位乳腺癌细胞到小鼠乳腺脂肪垫来研究肿瘤的生长。

三氯化铁诱发血栓形成小鼠模型上的颈动脉和肠系膜血管

三氯化铁诱导小鼠模型的血栓形成

3D全心脏心肌组织分析

通过轻型显微镜对小鼠急性心肌炎模型中白细胞浸润的定量可视化

制备和<em>体外</em>磁化的表征的miR-改性内皮细胞

小鼠外周动脉疾病模型中单核细胞归巢和肿瘤相关血管生成的活体显微镜观察

氯化铁诱导的犬颈动脉血栓形成: 一种大的血管损伤动物模型

石蜡内嵌组织中人与鼠中性粒细胞外陷阱的免疫荧光标记

Read Article