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Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
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Biologia
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JoVE Journal Biologia
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

DOI:
10.3791/65005-v

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08:07 min

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April 28, 2023

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, , , ,
1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL),Universidad de Buenos Aires, CONICET, 2Signalling Programme,The Babraham Institute

Capítulos

  • 00:05Introduction
  • 00:49PANC-1 Cell Preparation and Gemcitabine Treatment
  • 01:56Fixation, Permeabilization, and Blocking the PANC-1 Cells
  • 02:32Antibody Incubation
  • 03:57DAPI Staining, Montage, and Visualization of the Cells
  • 04:51LC3 Dots Quantification
  • 06:13Results: Effect of LC3 Immunofluorescence in Pancreatic Cell Lines
  • 07:26Conclusion

Summary

Tadução automática

Tadução automática

The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.

Tags

AutophagyLC3 ImmunofluorescencePancreatic CellsCell PreparationGemcitabine TreatmentCell Fixation And PermeabilizationPrimary Antibody IncubationFluorescent Imaging

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