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Capítulos
- 00:05Introduction
- 01:34Fabricating the Master Wafer and Preparing the Microfluidic Device
- 03:46Making the PDMS-Coated Glass Slide and Bonding the Microfluidic Device
- 05:24Surface Functionalization of the Microfluidic Device
- 06:48Liposome Production
- 07:33Results: Production of Monodispersed Liposomes and Subsequent pH-Triggered Liquid-Liquid Phase Separation of pLL/ATP Inside Liposomes
- 09:02Conclusion
Denne protokol beskriver octanol-assisteret liposomsamling (OLA), en mikrofluidisk teknik til generering af biokompatible liposomer. OLA producerer monodispergerede, mikron-størrelse liposomer med effektiv indkapsling, hvilket muliggør øjeblikkelig on-chip eksperimentering. Denne protokol forventes at være særlig velegnet til syntetisk biologi og syntetisk celleforskning.
Tags
On-chip Liposome AssemblyOLAMicrofluidic PlatformSynthetic BiologyArtificial CellsSelf-organizationCell-mimicking AssembliesMonodispersed LiposomesHigh-throughputEncapsulation EfficiencyBiological ReactionsVesicle DynamicsBiomolecular CondensatesMembrane InteractionsDrug ScreeningAntimicrobial TestingSurface FunctionalizationPhotolithography
