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Etablering, vedligeholdelse, differentiering, genetisk manipulation og transplantation af mus- og humane tårekirtelorganoider
JoVE Journal
Biologia
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JoVE Journal Biologia
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
DOI:

10:49 min

February 03, 2023

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Capítulos

  • 00:04Introduction
  • 00:39Organoid Derivation
  • 03:41Functional Swelling Assay
  • 04:45Organoid Electroporation
  • 06:36Orthotopic Transplantation of Human Lacrimal Gland Organoids
  • 08:17Results: Establishment, Genetic Modification, Differentiation, Functional Characterization, and Transplantation of Lacrimal Gland Organoids
  • 10:18Conclusion

Summary

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Denne protokol beskriver, hvordan man etablerer, vedligeholder, genetisk modificerer, differentierer, funktionelt karakteriserer og transplanterer lacrimalkirtelorganoider afledt af primært muse- og humant væv.

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