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Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

DOI:
10.3791/65062-v

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06:28 min

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February 17, 2023

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, ,
1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Capítulos

  • 00:04Introduction
  • 00:42Cell Culture
  • 01:44Isolation of Extracellular Vesicles
  • 03:14Western Blotting
  • 04:47Results: Characterization of Extracellular Vesicles
  • 05:35Conclusion

Summary

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The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

Tags

Keywords: Extracellular VesiclesEndotoxinLow-endotoxinCell CultureSupernatantUltracentrifugationFiltrationSW480SW620PBSFBSAseptic ProcedurePyrogenicLow Protein Binding

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