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分离来自癌细胞系的低内毒素含量细胞外囊泡
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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分离来自癌细胞系的低内毒素含量细胞外囊泡

DOI:
10.3791/65062-v

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06:28 min

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February 17, 2023

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, ,
1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Capítulos

  • 00:04Introduction
  • 00:42Cell Culture
  • 01:44Isolation of Extracellular Vesicles
  • 03:14Western Blotting
  • 04:47Results: Characterization of Extracellular Vesicles
  • 05:35Conclusion

Summary

Tadução automática

Tadução automática

拟议的方案包括有关如何在从细胞培养上清液中分离细胞外囊泡以及如何正确评估它们期间避免内毒素污染的指南。

Tags

Extracellular VesiclesEndotoxinLow-endotoxinCell CultureSupernatantUltracentrifugationFiltrationSW480SW620PBSFBSAseptic ProcedurePyrogenicLow Protein Binding

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