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Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
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Capítulos
- 00:04Introduction
- 01:12Fetus Tissue Excision
- 02:12Fetal Skeletal Muscle Decellularization
- 03:38Cell Culture in Decellularized Matrices
- 05:24Results: Fetal Muscle Tissue Protein Analysis
- 07:16Conclusion
In this work, a decellularization protocol was optimized to obtain decellularized matrices of fetal mouse skeletal muscle. C2C12 myoblasts can colonize these matrices, proliferate, and differentiate. This in vitro model can be used to study cell behavior in the context of skeletal muscle diseases such as muscular dystrophies.
