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1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research,Southwest Medical University, 2Department of Cardiology,the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 4Department of Pharmacology,University of Oxford
Capítulos
- 00:04Introduction
- 01:00Mouse Heart Harvest, Cannulation, and Perfusion
- 03:14Optical Mapping and Arrhythmia Induction
- 06:38Results: APD80 and CaTD80 Analysis at 10 Hz Before and After ISO Challenge
- 08:36Conclusion
Dit protocol introduceert dual-dye optische mapping van muizenharten verkregen van wildtype en knock-in dieren die zijn aangetast door catecholaminerge polymorfe ventriculaire tachycardie, inclusief elektrofysiologische metingen van transmembraanspanning en intracellulaire Ca2+ transiënten met hoge temporele en ruimtelijke resolutie.
