É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research,Southwest Medical University, 2Department of Cardiology,the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 4Department of Pharmacology,University of Oxford
Capítulos
- 00:04Introduction
- 01:00Mouse Heart Harvest, Cannulation, and Perfusion
- 03:14Optical Mapping and Arrhythmia Induction
- 06:38Results: APD80 and CaTD80 Analysis at 10 Hz Before and After ISO Challenge
- 08:36Conclusion
Ce protocole introduit une cartographie optique à double colorant des cœurs de souris obtenus à partir d’animaux de type sauvage et knock-in atteints de tachycardie ventriculaire polymorphe catécholaminergique, y compris des mesures électrophysiologiques de la tension transmembranaire et des transitoires intracellulaires Ca2+ avec une haute résolution temporelle et spatiale.
