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JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor
 

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

Article DOI: 10.3791/65085-v • 07:21 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 1, 2, 2, 1, 3, 1,4,5,6, 1,4
1Fraunhofer Project Center for Stem Cell Process Engineering, Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology, Saarland University, 6Facultad de Ciencias del Mar, Universidad Católica del Norte

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This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.

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Bioreactor Induced Pluripotent Stem Cells (iPSCs) 3D Culture Neuronal Differentiation Cell Yield Batch-to-batch Variation Cost Reduction Time Efficiency European Bank Of Induced Pluripotent Stem Cells Pre-cultivation Trypsin EDTA Solution Petri Dish Cell Detachment Feeder-free IPSC Maintenance Medium ROCK Inhibitor
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