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Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time

Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time

DOI:
10.3791/65149-v

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05:49 min

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March 24, 2023

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, ,
1Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy,KU Leuven

Capítulos

  • 00:04Introduction
  • 00:40Cell‐Based Electrical Impedance (CEI) Plate Preparation, Cell Seeding, and CEI Assay
  • 02:12Antiviral Compound Preparation and Virus Infection
  • 03:28Data Analysis
  • 03:57Results: Effect of Antiviral Compound and Zika Virus Replication on CEI Profiles
  • 05:22Conclusion

Summary

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Here, we demonstrate the use of cell-based electrical impedance (CEI) as a very easy and straightforward method to study Zika virus infection and replication in human cells in real time. Furthermore, the CEI assay is useful for the evaluation of antiviral compounds.

Tags

Cell-based Electrical ImpedanceReal-time Viral InfectionAntiviral CompoundsA549 CellsCEI AssayECIS DeviceVirus InhibitorsCell AdherenceCell Monolayer

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