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基于细胞的电阻抗平台，用于实时评估寨卡病毒抑制剂
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time
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基于细胞的电阻抗平台，用于实时评估寨卡病毒抑制剂

DOI:
10.3791/65149-v

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05:49 min

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March 24, 2023

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, ,
1Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy,KU Leuven

Capítulos

  • 00:04Introduction
  • 00:40Cell‐Based Electrical Impedance (CEI) Plate Preparation, Cell Seeding, and CEI Assay
  • 02:12Antiviral Compound Preparation and Virus Infection
  • 03:28Data Analysis
  • 03:57Results: Effect of Antiviral Compound and Zika Virus Replication on CEI Profiles
  • 05:22Conclusion

Summary

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在这里，我们展示了使用基于细胞的电阻抗（CEI）作为一种非常简单直接的方法来实时研究寨卡病毒感染和人类细胞中的复制。此外，CEI测定可用于评估抗病毒化合物。

Tags

Cell-based Electrical ImpedanceReal-time Viral InfectionAntiviral CompoundsA549 CellsCEI AssayECIS DeviceVirus InhibitorsCell AdherenceCell Monolayer

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