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拟南芥花粉管接收和半体外隔膜法 双受精的实时成像

JoVE Journal
Biologia

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JoVE Journal Biologia

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

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拟南芥花粉管接收和半体外隔膜法双受精的实时成像

DOI:

10.3791/65156-v

•

06:45 min

•

February 24, 2023

•

Nicholas J. Desnoyer, Ueli Grossniklaus

¹Department of Plant and Microbial Biology and Zurich-Basel Plant Science Center,University of Zurich

Capítulos

00:04Introduction
00:41Pollen Germination Medium Preparation
01:24Floral Staging of Septum Donors, Stigma Donors, and Pollen Donors
02:04Septum Dissection
03:20Stigma Dissection
03:55Imaging
04:46Results: Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization
06:02Conclusion

Summary

Tadução automática

在这里，我们描述了用于观察拟南芥花粉管引导和接收的半体外（SIV）方法的改进，该方法增加了胚珠的接受性。高通量SIV暨隔膜法可以与配子体标记系和遗传编码生物传感器相结合，以监测受精的动态过程。

Tags

Keywords: Arabidopsis Pollen Tube Double Fertilization Live Imaging Semi-in Vitro Ovule Septum Dissection Pistil Emasculation Pollination Microscopy

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