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Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs
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JoVE Journal Bioengenharia
Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

DOI:
10.3791/65158-v

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07:48 min

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May 05, 2023

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, , ,
1Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),University of Copenhagen, 2Department of Health Technology (DTU Health Tech),Technical University of Denmark, 3Department of Biotechnology and Biomedicine (DTU Bioengineering),Technical University of Denmark

Capítulos

  • 00:04Introduction
  • 00:46Self Healing Annealable Particle: Extracellular Matrix (SHAPE) Composite Preparation
  • 02:40Human Neural Stem Cell (hNSC) Bioink Preparation and Embedded 3D Printing
  • 06:19Results: Characterization of 3D Printed Human Neural Construct
  • 07:11Conclusion

Summary

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Tadução automática

This work describes a protocol for the freeform embedded 3D printing of neural stem cells inside self-healing annealable particle-extracellular matrix composites. The protocol enables the programmable patterning of interconnected human neural tissue constructs with high fidelity.

Tags

3D PrintingNeural ConstructsMicrogelExtracellular MatrixHydrogelSHAPEBiomaterialsAlginateCollagenHuman Neural Stem CellsNeurodegenerative DisordersParkinson’s Disease

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