In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

Journal

Biologia

JoVE Journal
Biologia

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JoVE Journal Biologia

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

DOI:

10.3791/652-v

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15:00 min

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January 25, 2008

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Ena Ladi, Paul Herzmark, Ellen Robey

¹Department of Molecular and Cell Biology,University of California, Berkeley

Capítulos

00:00Título
00:41Introduction
01:36Adoptive Transfer
02:51Dissection of Thymus
09:01Preparing Thymus for Imaging
10:232-photon Imaging
12:05Example of 2-photon Imaging Results
13:57Conclusion

Summary

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We present step-by-step instructions for the generation of neonatal chimeras as well as the dissection and preparation of the thymus for ex vivo imaging by 2-Photon Microscopy.

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

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Capítulos

Summary

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