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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

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Imunofluorescência Multiplex Combinada com Análise Espacial de Imagens para Avaliação Clínica e Biológica do Microambiente Tumoral

JoVE Journal
Pesquisa do Câncer

This content is Free Access.

JoVE Journal Pesquisa do Câncer

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Imunofluorescência Multiplex Combinada com Análise Espacial de Imagens para Avaliação Clínica e Biológica do Microambiente Tumoral

Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

DOI:

10.3791/65220-v

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01:36 min

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June 02, 2023

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Nicolas Huyghe, Elena Benidovskaya, Simon Beyaert, Aurélie Daumerie, Finoula Maestre Osorio, Frank Aboubakar Nana³, Caroline Bouzin, Marc Van den Eynde⁵

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

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Keywords: Multiplex Immunofluorescence Spatial Image Analysis Tumor Microenvironment Colorectal Cancer Metastatic Disease Immunotherapy Biomarkers RNA Sequencing Whole Exome Sequencing TCR Sequencing Circulating Cell-free DNA Analysis Fluorescence In Situ Hybridization Tyramide Signal Amplification Image Analysis Spatial Analysis Tumor Compartments Cell-cell Interactions

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