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Semi-Automated Isolation of Murine White Adipose Tissue

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Isolement semi-automatisé de la fraction vasculaire stromale à partir de tissu adipeux blanc murin à l’aide d’un dissociateur tissulaire

JoVE Journal
Biologia

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JoVE Journal Biologia

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

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Isolement semi-automatisé de la fraction vasculaire stromale à partir de tissu adipeux blanc murin à l’aide d’un dissociateur tissulaire

Semi-Automated Isolation of Murine White Adipose Tissue

DOI:

10.3791/65265-v

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02:39 min

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May 19, 2023

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Kaede Saito², Yuta Hiraike^2,3, Misato Oguchi, Toshimasa Yamauchi

¹Division for Health Service Promotion,The University of Tokyo, ²Department of Diabetes and Metabolic Diseases, Graduate School of Medicine,The University of Tokyo, ³The University of Tokyo Excellent Young Researcher Program,The University of Tokyo

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Keywords: Stromal Vascular Fraction Collagenase Digestion Adipocyte Differentiation Brown Adipocyte Beige Adipocyte White Adipocyte NFI Transcription Factor PPAR-gamma Energy Expenditure Obesity Diabetes

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Isolement semi-automatisé de la fraction vasculaire stromale à partir de tissu adipeux blanc murin à l’aide d’un dissociateur tissulaire

Isolement, digestion, filtration et placage cellulaire du tissu adipeux murin

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