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Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche
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Ex Vivo Protocol for Culturing Quiescent Muscle Stem Cells with Niche Components (Video) | JoVE
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Biologia
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JoVE Journal Biologia
Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence

Ex Vivo Protocol for Culturing Quiescent Muscle Stem Cells with Niche Components (Video) | JoVE

Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche

DOI:
10.3791/65433-v

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01:45 min

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June 02, 2023

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, , , , , ,
1Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France, 2Ecole nationale vétérinaire d’Alfort, IMRB, F-94700 Maisons-Alfort, France, 3EFS, IMRB, F-94010 Creteil, France, 4AP-HP, Hopital Mondor, Service d’histologie, F-94010 Creteil, France

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Keywords: Skeletal MuscleSatellite CellsNicheQuiescenceSingle-cell RNA SequencingFACSIn Vitro CultureMyofibersStem CellsEndothelial CellsFibro-adipogenic ProgenitorsRegenerationHomeostasisDisease

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