Journal
/
Bioquímica
/
Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays
/
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays

DOI:
10.3791/65718-v

•

01:09 min

•

January 12, 2024

•

,
1Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Tags

Biophysical AssaysTernary Complex FormationProteolysis Targeting Chimeras (PROTACs)Surface Plasmon Resonance (SPR)Biolayer Interferometry (BLI)Isothermal Titration Calorimetry (ITC)Brd4BD2VHLPPM1DCereblon (CRBN)KineticsThermodynamicsCooperativityHook Effect

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Isothermal Titration Calorimetry for Evaluating PROTACS-Induced Ternary Complex Formation

Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation

Surface Plasmon Resonance (SPR) for Evaluating PROTACS-Induced Ternary Complex Formation

Read Article