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Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation

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고분해능 구조 측정을 위해 Monolayer Graphene을 Cryo-Electron Microscopy Grid에 적용

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

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고분해능 구조 측정을 위해 Monolayer Graphene을 Cryo-Electron Microscopy Grid에 적용

Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation

DOI:

10.3791/66023-v

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01:38 min

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November 10, 2023

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Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis²

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Tags

Keywords: Cryo-electron Microscopy Graphene Monolayer Sample Preparation Particle Localization Air-water Interface Grid Surface Sample Concentration Background Noise Affinity Handles Cell Lysates Cryogenic Electron Microscopy Holey Carbon Foil Vitreous Ice Image Processing Near-atomic Resolution Sample Preparation Bottleneck Graphene-coated Grids

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