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Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

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Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

JoVE Journal
Biologia

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JoVE Journal Biologia

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

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Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

DOI:

10.3791/66085-v

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01:52 min

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January 05, 2024

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Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

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Keywords: Immunoprecipitation Protein-protein Interactions Anti-epitope Tag Affinity Gel Brown And Beige Fat PRDM16 NFIA PPARα ELK-1 Immune Cell Adipocyte Crosstalk Quantitative PCR Chromatin Immunoprecipitation Mass Spectrometry Protein Degradation HEK-293 Cells Polyethylenimine Protein Lysis Buffer Epitope Tag Affinity Gel Nuclear Extracts

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