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Live Cell Single-Molecule Imaging-Based Quantification of Protein Interactions

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Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

JoVE Journal
Biologia

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JoVE Journal Biologia

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

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Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

Live Cell Single-Molecule Imaging-Based Quantification of Protein Interactions

DOI:

10.3791/66169-v

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00:43 min

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January 05, 2024

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Shawn R. Yoshida², Shasha Chong

¹Division of Chemistry and Chemical Engineering,California Institute of Technology, ²Division of Biology and Biological Engineering,California Institute of Technology

Tags

Keywords: Single-molecule Measurement Protein Interaction Dynamics Biomolecular Condensates Liquid-liquid Phase Separation (LLPS)Single-particle Tracking (SPT)Binding Residence Time (RT)Protein-droplet Binding Oligomerizing Domain

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