É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:35Introduction
- 02:13Sacrifice, Dissection, and Digestion of Aplysia Abdominal Ganglion
- 04:37Dissection of Aplysia Bag Cells from Abdominal Ganglion and Plating
- 10:17Setting up Aplysia bag cells for live imaging
- 12:35Visualization of Growth Cone Morphology, Motility, and Cytoskeletal
- 15:48Conclusion
Aplysia californica neurons develop large growth cones in culture that are excellent for high-resolution imaging of growth cone motility and guidance. Here, we present a protocol for dissection and plating of Aplysia bag cell neurons as well as for setting up a chamber for live cell imaging.
Tags
Neuronal Cell CulturesAplysiaHigh-resolution ImagingGrowth ConesGuidance CuesDirectional MovementLive Cell ImagingProtein DynamicsTemporal ResolutionSpatial ResolutionF-actin DynamicsMicrotubule DynamicsSea Hare NeuronsMicromanipulationBiophysical MeasurementsCytoplasmic RegionsCytoskeletal SystemMicroinjectionGrowth Cone MotilityImaging Chamber
