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Analyzing LPS/ATP-Triggered Cell Death Mechanisms in THP-1 Cells

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LPS和ATP诱导的PMA分化THP-1巨噬细胞死亡及其验证

JoVE Journal
Biologia

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JoVE Journal Biologia

LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation

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LPS和ATP诱导的PMA分化THP-1巨噬细胞死亡及其验证

Analyzing LPS/ATP-Triggered Cell Death Mechanisms in THP-1 Cells

DOI:

10.3791/66831-v

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01:28 min

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May 03, 2024

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Huan Wang, Yuejia Lan, Cuiping Chen, Lu Yang, Jiayi Sun, Yong Zeng, Jiasi Wu

¹State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine, ²Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, ³Acupuncture and Tuina School,Chengdu University of Traditional Chinese Medicine

Tags

Cell Death Macrophage THP-1 Lipopolysaccharide (LPS)Adenosine Triphosphate (ATP)Pyroptosis Apoptosis Necroptosis Annexin V 7-Aminoactinomycin D (AAD)Flow Cytometry Scanning Electron Microscopy

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LPS和ATP诱导的PMA分化THP-1巨噬细胞死亡及其验证

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