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在体内2光子层钙成像对小鼠的2 / 3
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Biologia
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JoVE Journal Biologia
In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
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在体内2光子层钙成像对小鼠的2 / 3

DOI:
10.3791/681-v

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08:10 min

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March 13, 2008

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,
1Department of Neurology,University of California, Los Angeles

Capítulos

  • 00:00Título
  • 00:46Introduction
  • 01:15Injecting the Fluorescent Calcium Indicator Dye into the Cortex
  • 05:36Imaging Cortical Calcium Dynamics in vivo
  • 06:57Typical Example of In Vivo Calcium Imaging
  • 07:23Conclusion

Summary

Tadução automática

Tadução automática

要了解在大脑皮层的微型网络的动态，这是必不可少的，同时记录了大量的神经元活动。在活体双光子钙成像是唯一的方法，允许一个记录密集的人口与单细胞的分辨率神经元活动。

Tags

In Vivo2-Photon Calcium ImagingLayer 2/3MiceNetwork DynamicsMicrocircuitsNeocortexSimultaneous RecordingActivity Of NeuronsLarge Number Of NeuronsSingle-cell ResolutionCranial Imaging WindowFluorescent Calcium Indicator DyeTime Lapse Calcium ImagingIn-vivo Two Photon MicroscopeAstrocyte-specific DyesNetwork InteractionsSubpopulations Of Neurons

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