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Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
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JoVE Journal Biologia
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

DOI:
10.3791/686-v

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07:42 min

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April 02, 2008

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, , ,
1Contrast Agent Research Group at the Center for Molecular and Functional Imaging, Department of Radiology, University of California San Francisco

Capítulos

  • 00:01Título
  • 00:55Introduction
  • 01:41Labeling of Human Mesenchymal Stem Cells with Fluorescent Dye DiD
  • 03:12Labeling of Human Embryonic Stem Cells with the Fluorescent Dye ICG
  • 06:23Imaging Stem Cells in vivo
  • 06:59Conclusion

Summary

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Tadução automática

This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.

Tags

LabelingStem CellsFluorescent DyesNon-invasive DetectionOptical ImagingIn Vivo MonitoringEx Vivo LabelingIntravenous InjectionVisualizationTarget OrgansPathologiesHuman Mesenchymal Stem Cells (hMSC)Lipophilic Fluorescent Dye DiDHuman Embryonic Stem Cells (hESC)FDA Approved Fluorescent Dye Indocyanine Green (ICG)Uptake MechanismAdherenceDiffusionPhospholipid Cell Membrane BilayerLabeling EfficiencySerum-free MediaSerum-containing MediaTransfection Agent Protamine SulfateContrast Agent Uptake

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