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Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection
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Biologia
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JoVE Journal Biologia
Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection

Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection

DOI:
10.3791/700-v

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06:01 min

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May 14, 2008

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, ,
1Plant Pathology,Cornell University, 2Boyce Thompson Institute for Plant Research,Cornell University

Capítulos

  • 00:12Introduction
  • 02:18Microinjection of Aphids
  • 05:27Conclusion

Summary

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Aphids are effective transmitters of plant viruses. Aphid microinjection of virus, the procedure we will show you today, is a technique allowing researchers to inject virus directly into the hemocoel of the aphid, bypassing the gut, one of the 2 major barriers for virus transmission in a circulative manner. The same technique is also used to inject dsRNA for RNAi.

Tags

Physiological BarriersViral TransmissionAphidsMicroinjectionPotato Loafroll Virus (PLRV)LuteoviridaeGreen Peach AphidPlant PhloemHemolymphSalivary GlandPhysalis PlantsPLRV TransmissionStuntingInterveinal ChlorosisYellowing Of LeavesViral Transmission Prevention

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