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在体内的单细胞电穿孔内完整的大脑发育
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Biologia
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JoVE Journal Biologia
Single Cell Electroporation in vivo within the Intact Developing Brain
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在体内的单细胞电穿孔内完整的大脑发育

DOI:
10.3791/705-v

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13:31 min

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July 11, 2008

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,
1Brain Research Centre,University of British Columbia – UBC, 2Department of Cellular and Physiological Sciences,University of British Columbia – UBC

Capítulos

  • 00:00Título
  • 00:52Introduction
  • 02:13Fabrication and Loading of Micropipettes
  • 04:02Single-Cell Electroporation Technique
  • 09:16Screening for Successfully Electroporated Cells
  • 10:16In Vivo 2-Photon Imaging of Neurons Fluorescently Labeled by Single-Cell Electroporation
  • 12:46Conclusion

Summary

Tadução automática

Tadução automática

单细胞电（SCE）是一个专门的技术，允许交付到单个细胞内完整的组织，在体内的准备工作，包括的DNA或其他大分子。在这里，我们标准煤荧光染料或成爪蟾蝌蚪的完整的大脑内的神经细胞的质粒DNA的详细过程。

Tags

Single-cell ElectroporationIn VivoIntact Developing BrainDNA DeliveryMacromoleculesIndividual CellsExperimental ManipulationsCell-autonomous EffectsGlobal TreatmentsIn Vivo Imaging TechniquesFluorescent MarkersCellular MorphologyCell GrowthIntracellular EventsXenopus Laevis TadpolesVideo ArticleProcedure For SCEFluorescent DyePlasmid DNANeuronsAlbino Xenopus TadpoleYield OptimizationTwo-photon Fluorescence Imaging

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