Journal
/
Biologia
/
Trypsinizing and Subculturing Mammalian Cells
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

DOI:
10.3791/755-v

•

05:59 min

•

June 12, 2008

•

,
1Molecular Pathology Laboratory Network, Inc

Capítulos

  • 00:00Título
  • 00:37Introduction
  • 00:56Trypsinizig and Subculturing Mammalian cells from a Monolayer
  • 04:00Passaging Cells in Suspension Culture
  • 05:36Conclusion

Summary

Tadução automática

Tadução automática

As cells reach confluency, they must be subcultured or passaged. This video will demonstrate a procedure for subculturing both adherent and suspension cells.

Tags

TrypsinizingSubculturingMammalian CellsConfluencyPassagingMitotic IndexCell DeathDetachmentPrimary Culture VesselTrypsinizationMechanical MeansCell SuspensionReseedingFresh CulturesSecondary CulturesGrowthFeedingTertiary CulturesTime IntervalCell LineGrowth Rate

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Measurement of Heme Synthesis Levels in Mammalian Cells

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Read Article