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Trypsinizing和传代的哺乳动物细胞
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JoVE Journal Biologia
Trypsinizing and Subculturing Mammalian Cells
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Trypsinizing和传代的哺乳动物细胞

DOI:
10.3791/755-v

•

05:59 min

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June 12, 2008

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,
1Molecular Pathology Laboratory Network, Inc

Capítulos

  • 00:00Título
  • 00:37Introduction
  • 00:56Trypsinizig and Subculturing Mammalian cells from a Monolayer
  • 04:00Passaging Cells in Suspension Culture
  • 05:36Conclusion

Summary

Tadução automática

Tadução automática

当细胞达到汇合，他们必须传代或传代。此影片将展示壁和悬浮细胞传代的过程。

Tags

TrypsinizingSubculturingMammalian CellsConfluencyPassagingMitotic IndexCell DeathDetachmentPrimary Culture VesselTrypsinizationMechanical MeansCell SuspensionReseedingFresh CulturesSecondary CulturesGrowthFeedingTertiary CulturesTime IntervalCell LineGrowth Rate

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