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Trypsinizing en Steekenting zoogdiercellen
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JoVE Journal Biologia
Trypsinizing and Subculturing Mammalian Cells
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Trypsinizing en Steekenting zoogdiercellen

DOI:
10.3791/755-v

•

05:59 min

•

June 12, 2008

•

,
1Molecular Pathology Laboratory Network, Inc

Capítulos

  • 00:00Título
  • 00:37Introduction
  • 00:56Trypsinizig and Subculturing Mammalian cells from a Monolayer
  • 04:00Passaging Cells in Suspension Culture
  • 05:36Conclusion

Summary

Tadução automática

Tadução automática

Als cellen bereiken confluentie, moeten ze worden subcultuur of gepasseerd. Deze video zal aantonen dat er een procedure voor de subcultuur zowel hechtende en vering cellen.

Tags

TrypsinizingSubculturingMammalian CellsConfluencyPassagingMitotic IndexCell DeathDetachmentPrimary Culture VesselTrypsinizationMechanical MeansCell SuspensionReseedingFresh CulturesSecondary CulturesGrowthFeedingTertiary CulturesTime IntervalCell LineGrowth Rate

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