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Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
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Biologia
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JoVE Journal Biologia
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

DOI:
10.3791/789-v

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10:38 min

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June 27, 2008

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, , ,
1Department of Hematology and Oncology,Brigham and Women’s Hospital and Harvard Medical School, 2Department of Cell Biology and Genetics,Erasmus University Medical Center, 3Department of Medicine, Howard Hughes Medical Institute,Brigham and Women’s Hospital and Harvard Medical School

Capítulos

  • 00:00Título
  • 00:51Introduction
  • 02:04Dissection and Isolation of Early Hematopoietic Stem Cells
  • 07:19Dissociation and FACS Analysis
  • 09:57Conclusion

Summary

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This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.

Tags

IsolationEarly Hematopoietic Stem CellsMurine Yolk SacAGMMouse EmbryoBlood SystemFetal LiverBone MarrowMicrodissectionFlow Cytometric SortingPure PopulationStem Cell PoolFunctional DifferencesGenetic ModelsVideo DemonstrationFACS PlotFunctional AssaysColony AssaysBone Marrow Transplants

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