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小鼠卵黄囊和AGM早期造血干细胞的分离
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Biologia
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JoVE Journal Biologia
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
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小鼠卵黄囊和AGM早期造血干细胞的分离

DOI:
10.3791/789-v

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10:38 min

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June 27, 2008

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, , ,
1Department of Hematology and Oncology,Brigham and Women’s Hospital and Harvard Medical School, 2Department of Cell Biology and Genetics,Erasmus University Medical Center, 3Department of Medicine, Howard Hughes Medical Institute,Brigham and Women’s Hospital and Harvard Medical School

Capítulos

  • 00:00Título
  • 00:51Introduction
  • 02:04Dissection and Isolation of Early Hematopoietic Stem Cells
  • 07:19Dissociation and FACS Analysis
  • 09:57Conclusion

Summary

Tadução automática

Tadução automática

该视频显示如何微观剖析，从胚胎的卵黄囊和主动脉 - 性腺 - 中肾区，使用流式细胞仪检测造血干细胞进行排序。

Tags

IsolationEarly Hematopoietic Stem CellsMurine Yolk SacAGMMouse EmbryoBlood SystemFetal LiverBone MarrowMicrodissectionFlow Cytometric SortingPure PopulationStem Cell PoolFunctional DifferencesGenetic ModelsVideo DemonstrationFACS PlotFunctional AssaysColony AssaysBone Marrow Transplants

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