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In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
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Biologia
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JoVE Journal Biologia
In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

DOI:
10.3791/825-v

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07:07 min

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July 23, 2008

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,
1Division of Cardiovascular Medicine,Stanford University

Capítulos

  • 00:00Título
  • 01:10Introduction
  • 02:06Preparing Single Cell Suspension of Embryonic Stem Cells
  • 03:46Generating Hanging Drop Cultures
  • 04:39Transferring Hanging Drops to Ultralow Attachment Plates
  • 05:17Plating Embryoid Bodies onto Gelatin- Coated Plates
  • 06:12Conclusion

Summary

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This video demonstrates how to conduct in vitro differentiation of mouse embryonic stem cells to embryoid bodies using the hanging drop method.

Tags

In Vitro DifferentiationMouse Embryonic Stem CellsHanging Drop MethodStem Cell DivisionSpecialized Cell TypesCell-based TherapiesSuspension CultureEmbryoid BodiesAggregation Of ES CellsHanging Drop CulturePetri Dish Lid

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