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Human ES cells: Starting Culture from Frozen Cells

JoVE Journal
Biologia

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JoVE Journal Biologia

Human ES cells: Starting Culture from Frozen Cells

Human ES cells: Starting Culture from Frozen Cells

DOI:

•

02:47 min

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November 09, 2006

•

Erin Trish, John Dimos, Kevin Eggan

¹Department of Molecular and Cell Biology,Harvard

Capítulos

00:03Prepare media
00:17Take cells from freezer and thaw
01:58Start culture

Summary

Tadução automática

Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.

Tags

Human ES Cells Culture Frozen Cells Embryonic Stem Cell Line HuES Media Mouse Embryonic Fibroblast Feeder Cells Serum Cell Debris Liquid Nitrogen Storage -80C Freezer Thawing Freezing Media DMSO Differentiation Suspension Seeding MEF Plate Incubator Media Replacement

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