Journal
/
Biologia
/
全基因组阵列比较基因组杂交技术示范
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Please note that all translations are automatically generated. Click here for the English version.

全基因组阵列比较基因组杂交技术示范

DOI:
10.3791/870-v

•

16:37 min

•

August 05, 2008

•

, , , ,
1Department of Cancer Genetics,BC Cancer Research Centre, 2Deeley Research Centre,BC Cancer Agency, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency

Capítulos

  • 00:04Título
  • 01:33Day 1: DNA Quality
  • 03:21Probe Labeling
  • 06:43Day 2: Sample Clean-Up
  • 09:29Calculation of Incorporations
  • 10:55Array Hybridization
  • 12:44Array Washing
  • 15:34Array Scanning

Summary

Tadução automática

Tadução automática

这个视频是一个全基因组平铺路径阵列比较基因组杂交，扫描整个人类基因组只用25-100纳克的DNA，可以从多种来源，包括档案福尔马林固定材料隔离​​技术示范的杂交协议。

Tags

Array CGHComparative Genomic HybridizationGenetic AlterationsCancerGenetic DiseasesSMRT ArrayBAC ClonesDNA SegmentsGene DosageCompetitive HybridizationFluorescent DyesSeeGH SoftwareLog2 Signal Intensity RatioChromosomal PositionDNA Rearrangement Events

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/870?article_type=v

双整装原位杂交技术在早期鸡胚
check_url/pt/870?article_type=v

斑马鱼整装高分辨率双荧光原位杂交
check_url/pt/870?article_type=v

使用全山原位杂交分子和有机体生物学链接
check_url/pt/870?article_type=v

DNA微阵列：样品的质量控制，阵列杂交和扫描
check_url/pt/870?article_type=v

全山原位杂交E8.5，E11.5小鼠胚胎
check_url/pt/870?article_type=v

条形码酵母图书馆竞争力的基因屏幕
check_url/pt/870?article_type=v

罕见的基因变异检测从集资使用斯普林特测序
check_url/pt/870?article_type=v

全山RNA荧光<em在原位</em>杂交<em>果蝇</em>胚胎
check_url/pt/870?article_type=v

阵列比较基因组杂交（CGH阵列），用于检测基因拷贝数变异的
check_url/pt/870?article_type=v

从真核细胞染色体外的环状DNA基因组范围内的净化

Read Article