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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
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Biologia
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JoVE Journal Biologia
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

DOI:
10.3791/870-v

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16:37 min

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August 05, 2008

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, , , ,
1Department of Cancer Genetics,BC Cancer Research Centre, 2Deeley Research Centre,BC Cancer Agency, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency

Capítulos

  • 00:04Título
  • 01:33Day 1: DNA Quality
  • 03:21Probe Labeling
  • 06:43Day 2: Sample Clean-Up
  • 09:29Calculation of Incorporations
  • 10:55Array Hybridization
  • 12:44Array Washing
  • 15:34Array Scanning

Summary

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This video is a technical demonstration of the hybridization protocol for whole genome tiling path array CGH, which scans the entire human genome using only 25-100 ng of DNA that can be isolated from a variety of sources, including archival formalin fixed material.

Tags

Array CGHComparative Genomic HybridizationGenetic AlterationsCancerGenetic DiseasesSMRT ArrayBAC ClonesDNA SegmentsGene DosageCompetitive HybridizationFluorescent DyesSeeGH SoftwareLog2 Signal Intensity RatioChromosomal PositionDNA Rearrangement Events

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