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文化早期鸡胚体外（新文化）的方法
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Biologia
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JoVE Journal Biologia
Method for Culture of Early Chick Embryos ex vivo (New Culture)
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文化早期鸡胚体外（新文化）的方法

DOI:
10.3791/903-v

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08:45 min

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October 20, 2008

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1Center for Environmental and Genetic Medicine,Institute of Biosciences and Technology – Texas A&M Health Science Center , 2Center for Environmental and Genetic Medicine,Texas A&M University (TAMU)

Capítulos

  • 00:00Título
  • 00:37Introduction
  • 01:09Explanting the Embryo in Saline
  • 01:58Centering the Embryo on a Ring
  • 03:09Setting Up the Culture
  • 04:07Transferring the Culture to the Incubator
  • 05:02Fixing the Embryo
  • 07:35Representative Results/Outcome
  • 08:17Conclusion

Summary

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本视频演示了新的文化，鸡胚培养24小时以外的鸡蛋的方法。这种方法使研究的早期发展（原始连胜，以14索姆），鼠标E7 - 9对应一个时期。这项技术的应用，包括电，原位杂交和免疫组化。

Tags

Chick Embryo CultureEarly Embryonic DevelopmentBrain FormationNeural TubeSomiteHeart PrimordiaGene Function AnalysisGrowth Factor Coated BeadsFGFsBMPsWhole Mount In Situ HybridizationImmunohistochemistryExplantationGlass RingAlbumine PoolCulture Dish SealingHumid ChamberTroubleshooting Guide

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