É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:49Introduction
- 01:16Fixing and Dehydrating the Embryos
- 03:28Prehybridzation
- 05:15Hybridizing Embryos with DIG- and FITC- Coupled Probes
- 06:41DIG Antibody Incubation and Color Reaction
- 09:40FITC Antibody Incubation and Color Reaction
- 11:52Fixation and Processing
- 13:34Resultados
- 15:08Conclusion
This video demonstrates 2-color whole mount in situ hybridization, a method by which the spatial and temporal expression pattern of 2 different genes can be visualized in young chick embryos. This method was originally introduced by David Wilkinson, Domingos Henrique, Phil Ingham and David Ish -Horowicz.
Tags
Double Whole Mount In Situ HybridizationEarly Chick EmbryosEmbryonic DevelopmentGene ExpressionBrain DevelopmentNeural Tube FormationSomite FormationHeart Primordia Formation2-color Whole Mount In Situ HybridizationEmbryo DissectionParaformaldehyde FixationPrehybridizationProbe HybridizationDIG ProbeFITC ProbeOvernight HybridizationDIG Coupled AntibodyFITC Coupled AntibodyColor Reaction For DIG SubstrateColor Reaction With FITCPhotograph And SectioningTroubleshooting Guide
