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1Department of Cancer Genetics and Developmental Biology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Department of Pathology and Laboratory Medicine,University of British Columbia – UBC, 4Photography/Video Production, Multi-Media Services,BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,,University of British Columbia – UBC
Capítulos
- 00:06Introduction
- 02:06The MeDIP Protocol
- 02:25Materials
- 02:56DNA Preparation
- 04:52DNA Sonication
- 06:33Immunoprecipitation of Methylated DNA
- 13:51DNA Purification
- 19:27PCR Validation
- 21:09Credits
Cette vidéo montre le protocole pour immunoprécipitation ADN méthylé (MeDIP). MeDIP est une procédure de deux jours qui extrait sélectivement des fragments d'ADN méthylé à partir d'un échantillon d'ADN génomique en utilisant des anticorps avec une spécificité pour la 5-méthylcytosine (anti-5 MC).
Tags
Methylated DNA ImmunoprecipitationDNA Methylation PatternsEpigeneticsGene ExpressionNormal DevelopmentDiseaseMethylation ProfilesMeDIPMethylated DNA ExtractionAntibody ReactionImmunoprecipitated DNAInput DNAHeat DenaturationAnti-5’mC AntibodyMagnetic BeadsSecondary AntibodyAntibody ComplexSeparation Of Methylated DNANon-methylated DNA Removal
