É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Department of Cancer Genetics and Developmental Biology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Department of Pathology and Laboratory Medicine,University of British Columbia – UBC, 4Photography/Video Production, Multi-Media Services,BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,,University of British Columbia – UBC
Capítulos
- 00:06Introduction
- 02:06The MeDIP Protocol
- 02:25Materials
- 02:56DNA Preparation
- 04:52DNA Sonication
- 06:33Immunoprecipitation of Methylated DNA
- 13:51DNA Purification
- 19:27PCR Validation
- 21:09Credits
Это видео демонстрирует протокол для метилированной иммунопреципитации ДНК (MeDIP). MeDIP является двухдневной процедуры, которые селективно экстракты метилированных фрагментов ДНК из генома образцов ДНК с использованием антител со специфичностью для 5-метилцитозин (анти-5 MC).
Tags
Methylated DNA ImmunoprecipitationDNA Methylation PatternsEpigeneticsGene ExpressionNormal DevelopmentDiseaseMethylation ProfilesMeDIPMethylated DNA ExtractionAntibody ReactionImmunoprecipitated DNAInput DNAHeat DenaturationAnti-5’mC AntibodyMagnetic BeadsSecondary AntibodyAntibody ComplexSeparation Of Methylated DNANon-methylated DNA Removal
