Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Journal

Biologia

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

DOI:

10.3791/939-v

•

05:30 min

•

October 08, 2008

•

Austin L. Brown, Brandon E. Johnson, Miriam B. Goodman

¹Department of Molecular and Cellular Physiology,Stanford University , ²Department of Molecular and Cellular Physiology,Stanford University School of Medicine

Capítulos

00:02Título
00:33Introduction
01:04Pulling Pipettes
03:28Fire Polishing Pipettes
04:42Conclusion

Summary

Tadução automática

This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

•

•

•

Capítulos

Summary

Tadução automática

Tags

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes

Fabrication of Amperometric Electrodes

Brain Banking: Making the Most of your Research Specimens

Making MR Imaging Child’s Play – Pediatric Neuroimaging Protocol, Guidelines and Procedure

Making Gynogenetic Diploid Zebrafish by Early Pressure

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

Measurement of Cellular Chemotaxis with ECIS/Taxis

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Read Article