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Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures
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Biologia
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JoVE Journal Biologia
Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures

Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures

DOI:
10.3791/951-v

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07:43 min

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October 17, 2008

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,
1Department of Pediatric Oncology,Dana Farber Cancer Institute, 2Department of Neurobiology,Harvard Medical School

Capítulos

  • 00:00Título
  • 00:41Introduction
  • 01:08Setting up the Compartmented Chambers
  • 04:17Maintaining Dorsal Root Ganglia Neurons In Compartmented Cultures
  • 06:31Representative Results/ Outcome
  • 06:57Conclusion

Summary

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Here we describe the technique of preparing and maintaining compartmented chambers for culturing sensory neurons of the dorsal root ganglia.

Tags

PreparationMaintenanceDorsal Root Ganglia NeuronsCompartmented CulturesAxonal ProcessesTarget-derived Growth FactorsNeuronal SurvivalNeuronal FunctionNeurotrophinsSensory NeuronsRetrograde TransportSignaling EndosomesCulture SystemRobert CampenotSelective StimulationNeurodegenerative Disorders

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