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Method for Whole Mount Antibody Staining in Chick
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Biologia
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JoVE Journal Biologia
Method for Whole Mount Antibody Staining in Chick

Method for Whole Mount Antibody Staining in Chick

DOI:
10.3791/956-v

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10:13 min

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February 02, 2009

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,
1Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine,Texas A&M University (TAMU)

Capítulos

  • 00:00Título
  • 00:54Introduction
  • 01:24Fixing the Embryos
  • 02:39Preparing Embryos for Antibody Treatment
  • 04:04Antibody Incubation
  • 05:38Color Reaction
  • 06:48Embryo Processing for Photography and Histology
  • 08:18Representative Results
  • 09:30Conclusion

Summary

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This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.

Tags

Whole Mount Antibody StainingChick EmbryoEarly Embryonic DevelopmentAntibody ExpressionDeveloping BrainNeural TubeSomiteHRP Conjugated Secondary AntibodiesParaformaldehyde FixationInactivation Of Endogenous PeroxidasePrimary Antibody ExposureWashesSecondary Antibody IncubationPeroxidase ActivityDiaminobenzidine Substrate ReactionWax SectioningAntigen SitesJane DoddTom Jessell

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