É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:31Introduction
- 01:00Pulling and Pressure Polishing Pipettes
- 04:27Conclusion
This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.
Tags
Pressure-polishingPipettesPatch-clamp RecordingGlass PipettesShapingFabricatingNeuronal Cell BodiesGlass-blowingAir PressureHeatShape ModificationMicropipette PullerSoft GlassHard GlassPipette ResistanceTip Opening DiameterSoda Lime GlassBorosilicate GlassHigh-pressure ValveMicroforgeUltrasmall CellsSingle-channel RecordingBlunt ShapePerforated-patch Clamp Recording
