/

/

产后小鼠小脑颗粒神经元祖细胞和神经元的分离和培养

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

Please note that all translations are automatically generated. Click here for the English version.

产后小鼠小脑颗粒神经元祖细胞和神经元的分离和培养

DOI:

•

16:04 min

•

January 16, 2009

•

Hae Young Lee, Lloyd A. Greene, Carol A. Mason³, M. Chiara Manzini⁴

¹Department of Genetics and Development,Columbia University , ²Department of Pathology and Cell Biology,Columbia University , ³Department of Neuroscience,Columbia University , ⁴Department of Neurology, Beth Israel Deaconess Medical Center,Harvard Medical School

Capítulos

00:00Título
01:10Introduction
01:37Preparing for Cerebellar Dissection
03:15Cerebellar Dissection and Meninges Removal
06:01Cerebellar Dissection and Meninges Removal
09:29Percoll Gradient Separation
12:03Pre-plating and Plating
13:23Resultados
14:32Conclusion

Summary

Tadução automática

在这里，我们提出了一个方法来隔离和文化的小脑颗粒神经元祖细胞和产后小鼠小脑颗粒神经元。

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

禽流感胚胎瓣膜祖细胞的分离和培养

从小鼠胚胎肾肾区细胞的分离和培养

大鼠肝细胞的分离和小学文化

在文化序列富集的精原干细胞和祖细胞（SSCS）推导长期成年小鼠SSC线

分离新生小鼠心肌文化和

分离，培养和成年小鼠Cardiomyoctyes的功能分析

小鼠原代胰腺腺泡细胞的分离和培养

分离，培养和移植肌卫星细胞

从成年小鼠切牙牙源性上皮干细胞的分离和培养

隔离和鼠标主动脉内皮细胞原代培养

Read Article