JOVE JOURNAL

Biochemistry

Showing 121 - 132 in 724 videos.

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Gabrielle R. Budziszewski, M. Elizabeth Snell, Tiffany R. Wright, Miranda L. Lynch, Sarah E. J. Bowman²

¹National High-Throughput Crystallization Center,Hauptman-Woodward Medical Research Institute, ²Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences,The State University of New York

Journal (Biochemistry)

High-Throughput Protein Crystallization via Microdialysis

Tristan O. C. Kwan, Amy E. Danson, Pascelle Draper, Paul Reardon, Isabel Moraes

¹National Physical Laboratory, ²SWISSCI LTD

Journal (Biochemistry)

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Jitske Bak, Lisa Landskron, Thijn R. Brummelkamp, Anastassis Perrakis

¹Oncode Institute and Division of Biochemistry,Netherlands Cancer Institute

Journal (Biochemistry)

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Núria Crua Asensio, Stefano Rizzieri, Alessandro Cuomo, Gian Gaetano Tartaglia³, Elsa Zacco

¹Center for Human Technology,Italian Institute of Technology (IIT), ²Department of Experimental Oncology,European Institute of Oncology (IEO), ³Department of Biology and Biotechnologies ‘Charles Darwin’,Sapienza University of Rome

Journal (Biochemistry)

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

Shuo Wang, Stephan Nussberger

¹Biophysics Department, Institute of Biomaterials and Biomolecular Systems,University of Stuttgart

Journal (Biochemistry)

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Xinghui Song, Hao Shen, Yanwei Li, Yueting Xing, Jiajia Wang, Chun Guo, Yingying Huang, Jin Chen

¹Central Laboratory of Women’s Hospital,Zhejiang University School of Medicine, ²School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University, ³Core Facilities,Zhejiang University School of Medicine

Journal (Biochemistry)

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Sayan Dutta Gupta, Dae-Geun Song, SueJung Lee, Jae Wook Lee, Jin-Soo Park, Chrisostomos Prodromou, Cheol-Ho Pan

¹Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), ²School of Life Sciences,University of Sussex

Journal (Biochemistry)

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Isadora da Silva Zanzarini, Gustavo Barbosa, Larissa de Oliveira Prado, Ingrid Fatima Zattoni, Gustavo Da Paz, Ademir Luiz do Prado³, Waldemar Volanski, Marcos Dinís Lavarda, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Vivian Rotuno Moure, Glaucio Valdameri

¹Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, ²Graduate Program in Pharmaceutical Sciences,Federal University of Parana, ³Federal Institute of Parana

Journal (Biochemistry)

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Ewa Rejnowicz, Jack Wright, Margaret Veldman-Jones, Steven Harborne

¹Peak Proteins Ltd.

Journal (Biochemistry)

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Eliana M. Wolpaw*¹, Marissa L. Frenett*¹, Claudia A. Mak, Sloane M. Zwanger, Madushi Raththagala

¹Department of Chemistry,Skidmore College

Journal (Biochemistry)

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Artem Bonchuk², Nikolay Zolotarev, Konstantin Balagurov², Olga Arkova, Pavel Georgiev

¹Department of the Control of Genetic Processes, Institute of Gene Biology,Russian Academy of Sciences, ²Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology,Russian Academy of Sciences

Journal (Biochemistry)

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Sarah-Luisa Dülk, Silvia Rouskin

¹Department of Microbiology,Harvard Medical School

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